It is of paramount importance that individuals with potential exposure to HIV get tested. It is recommended to consult a healthcare professional as soon as possible to obtain information on appropriate testing methods and the correct timing for the test.

Nucleic Acid Tests (NAT, PCR) can generally detect HIV infection between 10 and 33 days after a risky exposure. These tests directly look for the genetic material of the virus.

Antigen/Antibody tests, usually performed using venous blood samples, can detect HIV infection between 18 and 45 days after a risky exposure. These tests look for antibodies the body produces against the virus (anti-HIV antibodies) and/or the virus's own antigens (p24 antigen). The ELISA test, commonly used for screening purposes, is a general test used for the diagnosis of many diseases and not exclusive to HIV. If the ELISA test is positive, more specific confirmation tests, such as Western Blot, are performed to confirm the diagnosis.

Polymerase Chain Reaction (PCR) tests, which determine the presence and quantity of viral genetic material, are used not only to aid in diagnosis in certain situations but also to monitor the effectiveness of HIV treatment and track viral load. Additional tests targeting the structural proteins of the virus may also be applied when deemed necessary.