This biological treatment method involves the complete surgical removal of the tumor-affected bone segment, carefully separating it from healthy bone tissue. The excised tumorous bone is then isolated from surrounding soft tissues and submerged in liquid nitrogen (cryotherapy) to devitalize it. This process effectively eradicates all cancerous cells while preserving the bone's structural integrity, even though its native cells are destroyed.

After devitalization, the bone is reimplanted into the patient's body. Simultaneously, a segment of the patient's own fibula, complete with its vascular supply, is harvested using microsurgical techniques and meticulously inserted into the devitalized bone. This living fibula promotes revascularization and regeneration within the mechanically strong, yet devitalized, bone over time.

As a result, patients can avoid limb amputation and maintain their quality of life, utilizing their own reconstructed bone, often without the need for prosthetic devices.