Diagnosis of tularemia primarily relies on a physical examination by a doctor. During this examination, the patient's symptoms, complaints, and the body regions affected by the disease are thoroughly assessed. To establish a definitive diagnosis, various laboratory tests may be requested after the physical examination.

The main diagnostic test methods that can be used for tularemia include:
* Blood test
* Biopsy
* Nasal or throat swab samples
* Pleural fluid analysis

Specifically, in cases of suspected pneumonic tularemia, a chest X-ray may also be requested to evaluate lung involvement and for the possible diagnosis of pneumonia.